AYU (An International Quarterly Journal of Research in Ayurveda)

PHARMACEUTICAL
Year
: 2019  |  Volume : 40  |  Issue : 4  |  Page : 262--272

Screening of herbal medicines for potential allopathic antidiabetic adulterants: An analytical study


Girish Pamidimarri1, Jayanthi Mathaiyan1, S Manikandan1, S Rajan1, Gitanjali Batmanabane2 
1 Department of Pharmacology, JIPMER, Puducherry, India
2 Department of Pharmacology, JIPMER, Puducherry; Director, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India

Correspondence Address:
Jayanthi Mathaiyan
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry - 605 006
India

Background: There are several reports worldwide on adulteration of herbal medicines (HMs) with allopathic drugs. In India, only a few studies have reported adulteration of HMs with antidiabetics and there are no systematic studies. Aims: To develop a rapid and validated method for detection of allopathic antidiabetic adulterants and to explore the extent of adulteration in HMs sold in South India. Materials and Methods: Standards and solvents were purchased from Sigma-Aldrich. Different brands of antidiabetic HM samples with manufacturing licenses were procured from dispensaries. Spiked drug free psyllium husk as solid and flask seed oil as liquid herbal matrices were used for method development. The spiked matrices with different concentrations were extracted with methanol and subjected to centrifugation. The supernatant was collected and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Isocratic elution was carried on a C18 column with 0.1% (v/v) formic acid:methanol (3:7, v/v) as a mobile phase. All drugs were monitored for two ion products in positive electrospray ionization mode using multiple reaction monitoring scans. Results: The retention time was 9 min. Limit of detection is 10 Pictograms (pg) for all analytes except for metformin, which was 370 pg. Recoveries of analytes range from 96% to 117%. Forty different brands of antidiabetic HMs were analyzed. Adulterant peaks were not observed in the mass chromatograms of HMs. Conclusions: A single-run method was developed by LC-MS/MS for the detection of proposed antidiabetics in HMs from licensed manufacturing units and online sold HMs across herbal dispensaries in Puducherry union territory, India. None of the HMs was found to be adulterated with proposed allopathic antidiabetic adulterants.


How to cite this article:
Pamidimarri G, Mathaiyan J, Manikandan S, Rajan S, Batmanabane G. Screening of herbal medicines for potential allopathic antidiabetic adulterants: An analytical study.AYU 2019;40:262-272


How to cite this URL:
Pamidimarri G, Mathaiyan J, Manikandan S, Rajan S, Batmanabane G. Screening of herbal medicines for potential allopathic antidiabetic adulterants: An analytical study. AYU [serial online] 2019 [cited 2022 Jan 18 ];40:262-272
Available from: https://www.ayujournal.org/article.asp?issn=0974-8520;year=2019;volume=40;issue=4;spage=262;epage=272;aulast=Pamidimarri;type=0