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CLINICAL RESEARCH
Year : 2013  |  Volume : 34  |  Issue : 1  |  Page : 63-69  

A comparative study of Dashana Samskara Choorna Pratisarana and Dashana Samskara paste application in the management of Sheetada (Gingivitis)


1 Senior Lecturer, Department of Shalya-Shalakya, Gampaha Wickramaraachchi Ayurveda Institute, University of Kelaniya, Yakkala, Sri Lanka
2 Associate Professor, Department of Shalakya Tantra, Institute for Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat, India
3 Professor and Head, Department of Periodontia, Government Dental College and Hospital, Jamnagar, Gujarat, India

Date of Web Publication23-Jul-2013

Correspondence Address:
K. P. P. Peiris
Senior Lecturer Gr -I, Department of Shalya-Shalakya, Gampaha Wickramaraachchi Ayurveda Institute, University of Kelaniya, Yakkala
Sri Lanka
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8520.115452

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   Abstract 

Sheetada is the early stage of periodontal diseases. This occurs due to negligence of oral hygiene, changing life-style, habits, and addictions. It is Kapha Rakta Pradhana Vyadhi. In modern dentistry papillary or marginal gingivitis can be correlated with Sheetada, on the basis of similarities in symptoms, involvement of anatomical structure, etiology and prognosis. The epidemiological studies conducted by American Academy of Periodontology shows that gingivitis of varying severities is nearly universal. It is estimated that over 80% of the world's population suffers from gingivitis. In this clinical study, 106 patients were registered among them 103 completed the treatment and were randomly divided by lottery method into two groups. In Group-A, Dashana Samskara paste local application on gums and in Group-B, Dashana Samskara Choorna Pratisarana on gums was given. After enrollment of the patients in the study cardinal symptoms of Sheetada (gingivitis) such as, Raktasrava, Krishnata, Prakledata, Mriduta, Mukhadaurgandhya, and also the objective criteria such as oral hygiene index, Gingival Index (GI-S), and Gingival Bleeding Index (GBI-S) were studied before and after the treatment.While considering comparative effect on subjective parameters such as Raktasrava, Dantamamsa Shiryamanata, Shotha and Chalata statistically significant results were obtained in Group-A than Group-B. In objective parameters such as, GI-S and GBI-S also showed statistically significant results in Group-A. Observations in follow-up study confirmed that the recurrence rate in the Group-Awas significantly lesser than the Group-B.

Keywords: Dashana Samskara Choorna , gingivitis, oral hygiene, Pratisarana, Sheetada


How to cite this article:
Peiris K, Rajagopala M, Patel N. A comparative study of Dashana Samskara Choorna Pratisarana and Dashana Samskara paste application in the management of Sheetada (Gingivitis). AYU 2013;34:63-9

How to cite this URL:
Peiris K, Rajagopala M, Patel N. A comparative study of Dashana Samskara Choorna Pratisarana and Dashana Samskara paste application in the management of Sheetada (Gingivitis). AYU [serial online] 2013 [cited 2020 Dec 1];34:63-9. Available from: https://www.ayujournal.org/text.asp?2013/34/1/63/115452


   Introduction Top


The Mukha (oral cavity) is considered to be one of the most important parts of the Urdhwajatru (part above the clavicles). Being the gateway of the alimentary canal, it reflects the body health. Acharya Sushruta classified the diseases of Mukha according to the seven subsites - Oshtha (lips), Dantamoola (gingiva and tooth supporting structures), Danta (teeth), Jihva (tongue), Talu (palate), Kantha (throat) and Sarvasara (oral mucosa).[1]

Sheetada is a disease described elaborately in Ayurveda as a type of Dantamoolagataroga (periodontal disease). The symptomatology of Sheetada can be considered as general marginal and papillary gingivitis, which may progress into periodontitis if not treated properly. Sheetada occurs due to vitiated Kapha and Rakta. The clinical features of the disease are Raktasrava (bleeding gums), Krishnata (discoloration of gums), Prakledata (moistness), Mriduta (spongyness), Shotha (gingival swelling), Mukhadaurgandhya (halitosis) at the initial stage. [2] In a later stage, Paka (suppuration), Dantamamsa Shiryamanata (gum recession) and Chalata (tooth mobility) may be seen. For the management of this disease systemic therapy, such as Nasya (insufflation), and local therapies such as Raktavisravana (blood letting), Pratisarana (local application), Gandoosha (mouthwash), Kavala (gargle), and Pralepana (paste) are advocated.

Gingivitis is a similar entity in modern dentistry and it was recognized as a clinical disorder in the mid-19 th century. [3] Gingivitis is non-destructive periodontal disease. If left untreated, it may progress to periodontitis, which is a destructive form.

Periodontal disease is widely regarded as the second most common oral disease world-wide after dental decay. [4] In the United States, it is prevalent in 30-50% of the population, but only about 10% have severe forms. [5] Several population based studies have shown its high prevalence and risk in systemic health. Modern research suggests that systemic health may be affected by oral hygiene more than previously recognized. For example, a recent review discussed possible etiological associations between periodontitis and cardiovascular disease in general and infective endocarditis specifically as well as rheumatoid arthritis, pneumonia, and preterm birth and low birth weight. [6]

The epidemiological studies conducted by American Academy of Periodontology show that gingivitis of varying severities is nearly universal and it is estimated that over 80% of the world's population suffers from gingivitis. [7] Among children and adolescents the incidence rate is 52.03%. In the 3 rd National Health and Nutritional Examination Survey, it was found that 50% of adults had gingivitis in at least 3-4 teeth. [8] In Gujarat, the prevalence of gingivitis was found to be 74.45%. [9]

In the present era prevailing treatment modalities, such as scaling and polishing, root planning, and gingivo plasty have their own limitations. Moreover, these modalities do not focus on regenerating and improving the health of the gingiva.

To overcome these problems, in Ayurvedic classics several treatment modalities such as Pratisarana, Gandoosha, and Kavala have been mentioned for the management of Sheetada.[10]

Among these, Pratisarana has been selected in this study and it is a kind of local application which mainly possesses therapeutic effects such as Shodhana (cleansing) and Ropana (growing). In Pratisarana, Choorna (fine powder) is mixed with lukewarm water to make a paste; the paste is taken on a fingertip and then massaged on the gums with mechanical pressure exerted in a specific direction. This process removes the food debris and plaque, which are the main causative factors of the disease. Further, Pratisarana helps to increase blood circulation and enhances gingival defense mechanism, giving strength to the gingival fibers to maintain the gingival, and the periodontal health.

Repeated advocacy of different Acharyas aroused an interest in a search for a better remedy for gingivitis from the medicinal heritage of Indian Materia Medica. An Ayurvedic formulation that is Kapha-Pitta Shamaka (pacifying Pitta and Rakta) and has Shothahara (anti-inflammatory), Krimighna (anti-microbial) and Rasayana (rejuvenation) properties is likely to be effective for the management of Sheetada.

Dashana Samskara Yoga formulation is documented in Ayurvedic literature. [11] It is a widely used classical formulation for treating all types of oral cavity disorders. It is mainly dominant with Katu (astringent) and Tikta Rasa (bitter taste); Laghu (light), Rooksha (rough), and Tikshna (stronge) Guna (properties); Katu Vipaaka; Sheeta Veerya (cold potency); and Kapha Pitta Shamaka Karma. Further, it's Shothahara (anti-inflammatory), Lekhana, Shodhana, Raktasthambhana (styptic), and Krimighna (anti-microbial) properties would help to remove the gingival pathology. Its Rasayana (immuno-promotive) property improves the gingival defence mechanism and helps to regenerate gingival tissues.

Although the Choorna (powder) form is a classical formulation, it has some demerits like short shelf life, greater chances of contamination, and inconvenience of application. Thus, patients were less likely to use it consistently and be satisfied with its benefits. To overcome these problems, a paste form of same the formulation was used. Pastes are widely used for dental disorders because they tend to be the most suitable and convenient formulation. It was in sterile form, and therefore has less likelihood of microbial contamination. It also has a longer shelf life.

After studying the classical references and results of previous research works, [12],[13] this present study was planned to compare the effect of Dashana Samskara Yoga clinically in paste and powder forms.


   Materials and Methods Top


Method of preparation paste

Nearly 30% hydroalcoholic extracts of each ingredient of Choorna (except Karpoora and Khatika) 25% w/w out of total Choorna was mixed with the base of gum acacia and sorbitol 48%. This paste was prepared at the ISO recommended pharmacy of K. P. Namboodiris Ayurvedics, R and D Laboratory, Vadakkekad, Thrissur.

In this Dashana Samskara paste preparation wet gum technique is used.

Process

  • Step 1: Gum base is dispersed thoroughly in water and transferred to the contra rotary mixer and added sorbitol.
  • Step 2: Added extracts of 1-8 and chalk powder and mixed thoroughly for 30 min until a homogenous mass is obtained.
  • Step 3: Added powdered Karpoora and continued mixing for 20 min.
After the paste formed, it was filled in 40 g aluminum tubes and packed under aseptic sterile conditions.

Patient's selection

Patients attending the Out Patient Dispensary of Department of Shalakya Tantra, with signs and symptoms of Sheetada (gingivitis) were registered irrespective of their sex, religion, occupation, education, etc., Total 106 patients were recruited for the study. An elaborative case taking proforma was specially designed for the purpose of incorporating all aspects of the disease in Ayurvedic and modern parlance. Patient's information sheet was prepared and Informedconsent was taken from all the registered patients for the trial.

Sampling technique

A total of 106 registered patients were divided into two groups (Group-A 54 patients, Group-B 52 patients) using the random sampling technique by lottery method to maintain the uniformity in both groups.

Grouping and posology

  • Group-A: Pratisarana with Dashana Samskara paste, 1 g twice a day
  • Group-B: Pratisarana with Dashana Samskara Choorna, 1 g twice a day
  • Kala: 15 min after meals
  • Duration: 1 month
  • Follow-up: 2 months after the completion of course of treatment.
Inclusion criteria

Patients having signs and symptoms of Sheetada (gingivitis) described as per the Ayurvedic and modern science of 16 to 60 years age were included. In this study, five subjective parameters as per Ayurvedic features and three objective parameters as per modern parameters were considered as inclusion criteria of all patients.

Exclusion criteria

  • Patients having any systemic diseases, i.e., diabetes mellitus, hypertension, hematological disorders, which causes gingivitis.
  • Patients using any other systemic drugs which may alter the results of the study.
  • Pregnant woman.
  • Patients below 16 years and above 60 years.
Investigations

Following investigations were carried out in order to rule out any systemic disease:

  • Routine hematological: Hb%, Total Count (TC), Differential Count (DC), Clotting Time (CT), Bleeding Time (BT), Platelet count and Erythrocyte Sedimentation Rate (ESR)
  • Biochemical: Blood sugar (R)
  • Urine: Routine and microscopic
  • Stool: Routine and microscopic
  • Microbiological examination: Gingival crevicular fluid swab culture before and after treatment of 20 patients in each group

   Method of Pratisarana Top


For Pratisarana Karma all the patients were advised to follow the given instructions, viz.:

  • Patients were advised to do Pratisarana for 2 times morning and evening after proper cleaning of mouth
  • Dashana Samskara Choorna should be taken in 1 g quantity and mixed with very little amount of luke warm water and make the Choorna in paste form
  • It should be taken on tip of the index finger and applied all over the gingiva smoothly with gentle pressure for 3-5 min in clockwise, round direction. Finally with slight pressure massage toward the gingival margin should be done and drug should remain on gingiva for 20-30 min
  • After that proper rinsing was advised with luke warm water
  • Same procedure was instructed for the paste and also application has been adopted by using tip of the finger.

   Instructions to the Patient Top


All the patients were advised to follow the instructions during therapy and in follow-up period:

  • Oral hygienic methods and their importance in the reversal of the disease were explained
  • Proper brushing by using soft brush 2 times a day morning and evening after meals by using "Bass" method was advised
  • Instructions regarding Ahara and Vihara were given, i.e. fibrous, non-sticky, less sweeten, etc., and proper mastication by using both sides
  • Proper mouth rinse after each meal/food item.
Assessment criteria



Objective criteria

  • Oral Hygiene Index (OHI-S)
  • Gingival Index (GI-S)
  • Gingival Bleeding Index (GBI-S).
Overall effect of therapy

The total effect of therapy was assessed considering the overall improvement in signs and symptoms.

  • Cured: 100% reliefs in the signs and symptoms and no recurrences during follow-up
  • Marked improvement: 76-99% relief in the signs and symptoms
  • Moderate improvement: 51-75% relief in the signs and symptoms
  • Mild improvement: 26-50% relief in the signs and symptoms
  • Unchanged: Up to 25% relief in the signs and symptoms
Statistical analysis

The information gathered on the basis of observations was subjected to statistical analysis. Chi-square test and unpaired Student "t-test" were carried out to observe the comparative effect of therapies on subjective and objective parameters.


   Observations and Results Top


Total 54 patients were registered in Group-A; among them 53 patients were completed the treatment. In Group-B, out of 52 registered patients, 50 patients completed the treatment.

In the present study, majority of the patients, i.e., 45.28% were from the age group of 31 to 45 years, 66.04% were females. Maximum, 49.06% of the patients were house wives. Maximum i.e. 61.32% patients were belonged to rural area, 34.91% were educated up to secondary education level. 48.11% patients were having Pitta-Kapha Deha Prakriti, 49.06% were taking Madhura Rasa dominant diet. Maximum i.e. 54.72% patients were having Mandagni.

Majority of the patients i.e., 50.9% were found to have moderate gingivitis and 40.57% were reported chronicity between 6 to 12 months.

Regarding oral hygienic measures, majority of patients, i.e., 90.57% were using tooth brush and 95.28% were using tooth paste as a cleansing material and 77.36% patients were cleaning their teeth once a day.

Maximum number of patients, i.e. 100% were having Raktasrava, Krishnata, Prakledata, Mriduta, and Dantamamsa Shiryamanata. Mukhadaurgandhya was presented in 90.57% of patients, Shotha was observed in 98.11%, Vedana in 60.38% and Chalata were reported in 23.58% patients. Before treatment the observations on objective parameters indicate that 42.45% patients had fair oral hygiene. On GI-S shows 50.95% patients with moderate gingivitis and 55.66% patients with moderate gingival bleeding.

Results of microbiological examination

Before starting the treatment, in Group-A, 15 samples were isolated with  Escherichia More Details coli bacilli and 5 samples with Streptococci. In Group-B, 13 samples were isolated with E. coli bacilli and 7 samples with Streptococci. After treatment in both the groups, all samples of gingival crevicular fluid were found free of pathogens.

Effect of therapies

Group-A:


In clinical features, 93.4% relief was found in Raktasrava, 100% in, 87.9% in Krishnata, 98.3% in Mriduta, 91.7% in Dantamamsa Shiryamanata, 100% in Prakledata and Paka, which are statistically highly significant (P < 0.001). In associated symptoms, Mukhadaurgandhya was relieved by 73.3%, Shotha by 94.9%, Vedana by 96.3% and Chalata by 91.7%, which are statistically highly significant (P < 0.001) [Figure 1] and [Figure 2].
Figure 1: Effect of therapies on clinical features I

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Figure 2: Effect of therapies on clinical features II

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On objective criteria, all objective parameters that is OHI-S, GI-S and GBI-S were improved by 77.3%, 90.6%, and 97.1% respectively and the results being statistically highly significant (P < 0.001) [Figure 3].
Figure 3: Effect of therapies on indices

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Group-B:

In clinical features, 77.3% relief was found in Raktasrava, in Krishnata 66.7%, in Prakledata 86.7%, in Mriduta 90.5% and Dantamamsa Shiryamanata 54.4%, which are statistically highly significant (P < 0.001). In associated symptoms, Mukhadaurgandhya was relieved by 59.3%, in Paka 96%, in Shotha 68.7% and in Vedana 97.1% relief was obtained, which are statistically highly significant (P < 0.001). Statistically insignificant improvement (P > 0.05) was found in Chalata only [Figure 1] and [Figure 2].

On objective criteria, OHI-S was improved by 72.2%, GI-S by 84.7% and GBI-S was improved by 88.2%, which are statistically highly significant at the level of P < 0.001 [Figure 3].

Comparative effect of therapies

Effects of Dashana Samskara paste and Choorna on chief complaints of Sheetada (gingivitis) shows that, statistically significant difference was obtained in four of the chief complaints that is Raktasrava, Dantamamsa Shiryamanata, Shotha, and Mukhadaurgandhya in Group-A, with comparison to Group-B. Data reveals that Group-A has been shown better effect on these clinical features than Group-B. The comparative effect on objective parameters reveals that Group-A shown statistically significant difference to that of Group-B. In two indices that is GI-S and GBI-S, the results were significant statistically with the P value at less than 0.05 level.

Comparative effect was obtained in GBI-Sand GI-S, which was statistically significant at the level of P < 0.01.However, in OHI-S it was statistically insignificant at the level of P > 0.10.

During the clinical study period, no any adverse effect was observed in any of the groups. This indicates that Dashana Samskara Yoga is safe and non-toxic in both the forms.

Clinical observations of patients after follow-up

After completion of the clinical trial of 1 month, the patients were followed-up for further 2 months in both groups. During this period, some patients have reported the recurrence or aggravations of the complaints. In Group-A, during follow-up study 19% patients were reported with altered OHI, followed by 2% patients reported with altered GI-S as well as GBI-S. In Group-B, maximum number of patients that is 50% were reported with altered GBI-Sduring follow-up period followed by altered GI-S in 24% patients and altered OHI-S was found in 40% patients.

Overall effect of therapy

In Group - A, 9.43% patients were cured, 79.25% patients had marked improvement and 9.43% patients were moderately improved followed by one patient was observed under mild improvement category. In Group - B, 2% patients were found under cured category, 58% patients in marked improvement, 32%) patients in moderate improvement and 8% patients were recorded in mild improvement category. None of the patients in both the groups were found under unchanged category [Figure 4].
Figure 4: Over all effect of therapies

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   Discussion Top


Gingivitis can occur at any age group. The epidemiological studies show that the prevalence rate is high among children and adolescences, [14] because of age related changes and lack of awareness. In this study, majority of patients (42.28%) were reported in the age group of 31-45 years that is the late phase of adolescence. Data clearly implies that above group of patients were under sub-clinical stage during their adolescence age and presented with Sheetada in our OPD in the late adolescence phase.

Global studies show that prevalence of gingivitis is more common among males. [15] However, maximum patients observed in the present study were females. This may be because of general trend of the female patients attending the hospital. As day time male patients may be engaged with their professional duties. Another factor may be females are careless about their oral health because of lack of time and awareness. In this study, among female patients, 57% were under premenopausal stage. It also could be the reason that majority of patients were females because studies also claim that hormonal imbalance have a direct role to play in the pathogenesis of gingivitis. [16]

It was observed in the present clinical study that maximum numbers of patients were belonged to rural area. This finding is supported by several previous research works. [17] They have proved that in general, the prevalence and severity of periodontal disease are slightly higher in rural areas than in urban areas. It may be due to lack of awareness and poor oral hygienic practice. The previous studies show that periodontal disease is inversely related to increasing levels of education. [18]

Maximum that is 48.11% of patients registered in this study, were having Kapha-Pitta Prakriti (constitution) and all of them presenting with moderate grade of gingivitis. Owing to the involvement of Kapha Dosha and Rakta Dhatu in Sheetada, Kapha-Pitta Prakriti is more prone to this disease due to the Aashrayashrayi Bhava. The majority of the patients were having moderate gingivitis, which indicates that maximum patient's gingival status can be regained to normal condition with proper management with early diagnosis.

It is a known fact that general condition of GIT favors the normal nourishment and health of the body tissues. It was observed that majority of the patients i.e., 54.72% were having Avara Jarana Shakti (low digestive power) and Mandagni (low biological fire). These may favor the formation of Ama (undigestive food) and initiation of Samprapti (aetio patogenesis) of Sheetada.

Present study shows that majority of patients had chronicity between 6 months and 12 months. It indicates that the disease is chronic in nature. Because of patient's unawareness of their oral health, they were not able to detect bleeding from gums as early symptom.

Nearly 54% of patients presented with fair oral hygiene. This may be due to lack of awareness of oral hygienic measures. [19] Maximum patients were using brush as a cleansing tool. This reveals that in this modern era it is a common tool for cleansing teeth. It is proved that with brush one can clean the interdental areas, pits, and fissures on the teeth. However, most important thing is which method of brushing is used. In this study, maximum patients were using horizontal brushing pattern. It is an improper pattern by which debris at inter dental area, pits, and fissure areas and more or less at cervical areas may not be removed, which plays major role in initiation or progression of the disease, not only that, but also improper technique and hard bristles damages the gingiva and its frequent use leads to increased friction leading to gingivitis or it may injure the enamel and cause abrasion of tooth surface. Nearly 95.28% patients were using tooth paste as a cleansing material. It indicates the changed trend of cleaning material, particularly in urban area, which prevents the gums and teeth from microbes. Because of the traditional Datuna (tooth stick) and tooth powder are not much in practice in urban area.

Nearly 77.36% of the patients found cleaning their teeth only once in a day, in morning and before breakfast. It indicates lack of importance of oral hygiene after taking food. It results in bacterial growth at the place of food collection resulting in dental plaque.

Maximum patients were vegetarians i.e., 83.02% who were taking Madhura Rasa dominant diet that is 49.06%. Protein rich diet is mainly responsible for the integrity of the periodontium and plays main role in the promotion and maintenance of gingival and periodontal health. Majority of vegetarian diets are rich in carbohydrates and fibrous content. It shows that lack of proteinous diet may have a role to play in gingival disorders. Madhura Rasa is responsible for vitiation of Kapha Dosha leads to initiation of Samprapti of Sheetada. Further, in modern dentistry, it is mentioned that sweet food items more frequently initiates dental plaque formation. [20]

Comparative effect of therapy on clinical features

Although considering comparative effect on clinical features such as Raktasrava, Dantamamsa Shiryamanata, Shotha and Chalata statistically significant results were obtained. This was confirmed that, Group-A Dashana Samskara paste has been shown better results in above clinical features than Dashana Samskara Choorna. Though maximum ingredients of both forms of Dashana Samskara Yoga have same pharmacodynamic properties such as Katu (pungent), Tikta (bitter), Kashaaya (astringent) Rasa (taste), Laghu (light), Ruksha (rough), Tikshna (sharp) Guna (properties), Sheeta Veerya, and Kapha Pitta Shamaka in nature predominant with Srotoshodhana, Lekhana, Sthambhana (checking), Krimighna and Shothahara actions and also with proven pharmacological actions such as anti-inflammatory, analgesic, styptic, and anti-microbial activities. However, paste has been shown more effect, which may be due to its quick absorption power enhanced by hydro-alcoholic nature with fineness, more penetrating capacity and equally dispersing ability on gingival epithelium.

Comparative effect of therapy on Indices

On assessing comparative effect objectively on the basis of improvement in all three indices, only GI-S and GBI-S have been shown statistically significant improvement at the level of P < 0.01. It was confirmed that Group-A shows better improvement in GI-S and GBI-S than Group-B. It may be due to quick absorption power, equally dispersing ability on gingival epithelium like qualities in paste and synergistic action of the paste base.


   Conclusion Top


Dashana Samskara Yoga in Choorna and paste form found to be effective in treating the features such as Raktasrava, Mriduta, Krishnata, Prakledata, Shotha, Dantamamsa Shiryamanata, and Mukhadaurgandhya. Paste formulation showed comparatively better results in the features such as Raktasrava Shotha, Dantamamsa Shiryamanata, and Chalata. During follow-up, the recurrence rate was minimum seen in the patients treated with paste formulation. Both the forms were found to be effective in controlling the oral microbes.

Recurrence of Sheetada (gingivitis) was minimal among the patients who were treated with the paste form of Dashana Samskara Yoga. Evidence drawn from clinical and follow-up studies have been confirmed that Dashana Samskara paste is more effective and appropriate to control the disease "Sheetada" in comparison to Choorna.

 
   References Top

1.Sushruta, Sushruta Samhita, Nidana Sthana, 16/3. In: Yadavji Trikamji Acharya, editor. 4 th ed. Varanasi: Chaukhambha Orientalia; 2008.  Back to cited text no. 1
    
2.Ibid, Sushruta Samhita, Nidana Sthana 16/13.  Back to cited text no. 2
    
3.Carrenza N. Clinical Periodontology. 8 th ed. Bangalore: Prism Books (Pvt.) Ltd.; 1996. p. 6.  Back to cited text no. 3
    
4.WHO. Technical Report Series, Recent Advances in Oral Health. Geneva: World Health Organisation; 1995.  Back to cited text no. 4
    
5.Wiebe CB, Putnins EE. The periodontal disease classification system of the American academy of periodontology - An update. J Can Dent Assoc 2000;66:594-7.  Back to cited text no. 5
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6.Holmstrup P, Poulsen AH, Andersen L, Skuldbøl T, Fiehn NE. Oral infections and systemic diseases. Dent Clin North Am 2003;47:575-98.  Back to cited text no. 6
    
7.Available from: http://www.quantumhealth.com/news/gingivitis. [Retrieved on 2009 Jun 15].  Back to cited text no. 7
    
8.Winn DM, Johnson CL, Kingman A. Periodontal disease estimates in NHANES-III: Clinical measurement and complex sample design issues. J Periodontol 2005;76:1406-19.  Back to cited text no. 8
    
9.Dhar V, Jain A, Van Dyke TE, Kohli A. Prevalence of gingival diseases, malocclusion and fluorosis in school-going children of rural areas in Udaipur district. J Indian Soc Pedod Prev Dent 2007;25:103-5.  Back to cited text no. 9
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10.Sushruta, Sushruta Samhita, Chikitsa Sthana, 22/11-12. In: Yadavji Trikamji Acharya, editor. 4 th ed. Varanasi: Chaukhambha Orientalia; 2008.  Back to cited text no. 10
    
11.Shri Govind Das, Bhaisajyaratnavali-Vidyotini Hindi Commentary Mukha Roga Chikitsa Prakarana, 61/97-98. Analysis with Appendixes: Keyathi Sansthanam. Varanasi: Chaukambha Sanskrit Santhan; 2005.  Back to cited text no. 11
    
12.Dexit V. Standerdization and quality control aspects of Dashana Samskara Choorna: An Ayurvedic formulation. Thesis. Gujarat Ayurved University; 2006.  Back to cited text no. 12
    
13.Unadhkat R, Manjusha R. A clinical study on Sheetada w.s.r. to gingivitis. Thesis. Gujarat Ayurved University; 2006.  Back to cited text no. 13
    
14.Hugoson A, Koch G, Rylander H. Prevalence and distribution on gingivitis - Periodontitis in children and adolescents epidemiological data as a base for risk group selection. Swed Dent J 1981;5:91-103.  Back to cited text no. 14
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15.Furuta M, Ekuni D, Irie K, Azuma T, Tomofuji T, Ogura T, et al. Sex differences in gingivitis relate to interaction of oral health behaviors in young people. J Periodontol 2011;82:558-65.  Back to cited text no. 15
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16.Reddy S. Essentials of Clinical Periodontology and Periodontics, 2 nd ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd.; 2008. p. 102-3.  Back to cited text no. 16
    
17.Kelly JE, Van Kirk LE. Periodontal Disease in Adults, United States 1960-1962. Washington, DC: U.S. Public Health Service, U.S. Department of Health Education and Welfare, National Centre for Health Statistics, Publications No. 1000, Series 11, No. 12; 1966.  Back to cited text no. 17
    
18.Oliver RC, Brown LJ, Loe H. Variations in the prevalence and extent of periodontitis. J Am Dent Assoc 1991;122:43-8.  Back to cited text no. 18
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19.Sayegh A, Dini EL, Holt RD, Bedi R. Oral health, sociodemographic factors, dietary and oral hygiene practices in Jordanian children. J Dent 2005;33:379-88.  Back to cited text no. 19
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20.Reddy S. Essentials of Clinical Periodontology and Periodontics, 2 nd ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd.; 2008. p. 95.  Back to cited text no. 20
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4]



 

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    Abstract
   Introduction
    Materials and Me...
    Method of Pra...
    Instructions to ...
    Observations and...
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    References
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