AYU (An International Quarterly Journal of Research in Ayurveda)

PHARMACOLOGICAL STUDY
Year
: 2017  |  Volume : 38  |  Issue : 2  |  Page : 171--178

Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats


Shrirang Jamadagni1, Pallavi Shrirang Jamadagni1, Rajendra Kumar Singh1, Sachchidanand Upadhyay1, Sudesh N Gaidhani2, Jayram Hazra1 
1 Department of Pharmacology, Central Ayurveda Research Institute of Drug Development, Kolkata, West Bengal, India
2 Department of Pharmacology, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, New Delhi, India

Correspondence Address:
Dr. Shrirang Jamadagni
Central Ayurveda Research Institute of Drug Development, 4 CN Block, Sector 5, Bidhananagar, Kolkata - 700 091, West Bengal
India

Context: Makaradhwaja is a Kupipakwa Rasayana. Since it contains two heavy metals, namely mercury and gold, it is essential to evaluate its safety. Hence, the present study was undertaken with an objective to evaluate toxicity and target organ of toxicity of Makaradhwaja if so. Aims: The objective was to evaluate toxicological profile, the target organ of toxicity and to find no observed effect level (NOEL) or no observed adverse effect level (NOAEL) in rats after oral administration for ninety consecutive days. Materials and Methods: Makaradhawaja preparation was administered to male and female Wistar rats for ninety consecutive days at 2.7, 13.5, and 27 mg/kg body weight. All relevant biochemical and hematological changes were observed. At termination, all the rats were sacrificed and necropsy was performed. Histopathological evaluation was also performed. Statistical Analysis Used: Dunnett's test followed by analysis of variance. Results: There was a significant increase in high-dose group kidney weight of both sexes which could not be correlated with histopathology findings and serum biochemistry. Therefore, the change was not considered as an adverse effect. Conclusions: The dose level 27 mg/kg of Makaradhwaja was found as NOAEL and dose level 13.5 mg/kg of Makaradhwaja was found as NOEL.


How to cite this article:
Jamadagni S, Jamadagni PS, Singh RK, Upadhyay S, Gaidhani SN, Hazra J. Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats.AYU 2017;38:171-178


How to cite this URL:
Jamadagni S, Jamadagni PS, Singh RK, Upadhyay S, Gaidhani SN, Hazra J. Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats. AYU [serial online] 2017 [cited 2019 Nov 20 ];38:171-178
Available from: http://www.ayujournal.org/article.asp?issn=0974-8520;year=2017;volume=38;issue=2;spage=171;epage=178;aulast=Jamadagni;type=0