Login   |  Users Online: 1225 Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
Search Article 
  
Advanced search 
   Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts
PHARMACOLOGICAL STUDY
Year : 2018  |  Volume : 39  |  Issue : 4  |  Page : 239-242

In vitro anti-inflammatory activity of Ficus racemosa L. bark using albumin denaturation method


1 Department of Bio-Medical Sciences, School of Biomedical Science and Physiology, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, UK
2 Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Colombo, Sri Lanka
3 Department of Alternative Medicine, Institute of Ayurveda and Alternative Medicine, Panadura, Sri Lanka
4 Department of Pre-Clinical Sciences, Faculty of Medicine, General Sir John Kotelawala Defence University, Colombo, Sri Lanka

Correspondence Address:
Dr. Nishantha Kumarasinghe
Department of Pre-Clinical Sciences, Faculty of Medicine, General Sir John Kotelawala Defence University, Colombo
Sri Lanka
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ayu.AYU_27_18

Rights and Permissions

Introduction: In Ayurveda, many natural plant compounds are used to inhibit inflammatory pathways for centuries with less side effects. Different parts of Ficus racemosa L. (Udumber) plant are used in Ayurveda for many diseases. However, few studies have been conducted to evaluate pharmacological activities of F. racemosa. Objective: The objective of the study was to in vitro analyze anti-inflammatory property of F. racemosa bark using albumin denaturation activity. Methodology: F. racemosa bark extraction was performed using cold water and hot water. The concentration gradient of extracts was prepared using egg albumin and phosphate-buffered saline. The extract was incubated in a water bath at 37°C for 15 min and was heated at 70°C for 5 min. One nonsteroidal anti-inflammatory drug and one steroid were used as reference drugs. The percentage inhibition of protein denaturation was calculated. Results: The inhibition rate of egg albumin denaturation for water extraction increased gradually with concentration. Significantly higher inhibition was showed in hot water extracts than cold water extracts at the concentration of 0.01 μg/ml and 0.1 μg/ml. In addition, the inhibition rate of water extraction was significantly higher than the reference drugs (P < 0.05). Conclusion: Anti-inflammatory activity increases with the concentration of F. racemosa bark. Furthermore, the action of this plant is significantly higher than the reference drugs.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed418    
    Printed3    
    Emailed0    
    PDF Downloaded63    
    Comments [Add]    

Recommend this journal