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PHARMACEUTICAL STUDY
Year : 2018  |  Volume : 39  |  Issue : 4  |  Page : 220-225

Natural stains Zingiber officinale Roscoe (ginger) and Curcuma longa L. (turmeric) – A substitute to eosin


Department of Oral Pathology and Microbiology, SDM College of Dental Sciences and Hospital, Dharwad, Karnataka, India

Correspondence Address:
Dr. Kaveri Hallikeri
Department of Oral Pathology and Microbiology, SDM College of Dental Sciences and Hospital, Dharwad - 580 009, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ayu.AYU_232_17

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Background: Stained slides are of the utmost source of information in pathology. The routine stains utilize synthetic dyes which pose various health hazards which necessitate the importance of natural extracts as a histological stain. Aims: The aim of this study is to compare the efficacy of ginger and turmeric extracts as histological dyes in tissues keeping eosin as standard. Objectives: The objective of this study is to compare the staining potential, intensity, specificity, and shelf life of ginger and termuric extract dyes. Materials and Methods: Extracts of fresh rhizomes of Zingiber officinale Roscoe and Curcuma longa L. were obtained by dissolving them in alcohol, which was used to stain sections of 25 cases. Observer 1 compared the staining intensity, while observers 2 and 3 assessed preset parameters at regular intervals and subjected to Kappa statistics and Mann–Whitney U-test. Results: Z. officinale and C. longa solutions had pH 4–5 and temperature 24°C–27°C. Z. officinale showed better staining intensity and specificity to the cytoplasm and basic components of connective tissue as compared to C. longa. Crispness (P = 0.01) and background staining (P = 0.05) showed a significant difference. Shelf life of Z. officinale was better than C. longa. Conclusion: Z. officinale gave a better staining than C. longa and was found to be closest to eosin and is easily available, cost-effective and biodegradable.


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