|Year : 2012 | Volume
| Issue : 3 | Page : 444-446
A comparative analytical study of Prasarani [Merremia tridentata Hallier. f. and Paederia foetida Linn.]
N Rajashekhara1, P Vasanth2, D Vijaya Kumar3
1 Professor and Head, Department of Dravyaguna Vijnan, P.G. Unit, K.V.G. Ayurveda Medical College, Sullia, Karnataka, India
2 Reader and Head, Department of Dravyaguna Vijnan, P.G. Unit, Dr. B.R.K.R. Government Ayurveda College, Erragada, Hyderabad, Andhra Pradesh, India
3 Principal Scientist, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Andhra Pradesh, India
|Date of Web Publication||16-Mar-2013|
Agrahara House, Post Subrahmanya - 574238, D.K. District, Karnataka
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Prasarani is one of the drugs used in Vata Rogas such as Amavata, Avabahuka, etc. Among the different source plants of Prasarani, the plant Merremia tridentata Haller.f. is mostly used in South India and the plant Paderia foetida Linn. in North India, hence taken in the present work for comparative analytical study. It was observed that there is a common constituent (having 350 mm absorbance maxima) present in both the drug samples indicating that both works on a similar disease. It was also found that the whole plant powder sample of P. foetida has more number of constituents than that of M. tridentata which indicates P. foetida may have a better efficacy than M. tridentata.
Keywords: HPLC , Merremia tridentata, Paederia foetida, phytochemistry, Prasarani
|How to cite this article:|
Rajashekhara N, Vasanth P, Kumar D V. A comparative analytical study of Prasarani [Merremia tridentata Hallier. f. and Paederia foetida Linn.]. AYU 2012;33:444-6
|How to cite this URL:|
Rajashekhara N, Vasanth P, Kumar D V. A comparative analytical study of Prasarani [Merremia tridentata Hallier. f. and Paederia foetida Linn.]. AYU [serial online] 2012 [cited 2020 Feb 18];33:444-6. Available from: http://www.ayujournal.org/text.asp?2012/33/3/444/108864
| Introduction|| |
Prasarani is one of the drugs used for Vata Rogas such as Amavata, Avabahuka, etc. References about Prasarani are found in Charaka Samhita,  Sushruta Samhita,  Ashtanga Sangraha,  Nighantus, etc.
Two species are being used as Prasarani mostly in South India and North India , viz,
Therefore the present study has been undertaken to compare both the above drugs Analytically.
- Merremia tridentata Hallier. f. (Convolvulaceae) [Figure 1].
- Paederia foedita Linn. (Rubiaceae) [Figure 2].
| Materials and Methods|| |
Aim of the study: To compare the the plants Merremia tridentata Hallier. f. and Paederia foetida Linn. under following parameters:-
- Phytochemical Review
- High Performance Liquid Chromatography (HPLC) Analysis
- Colour Study
- Determination of Ash Values
- Merremia tridentata Hallier. f. - Flavanoidis:- Diosmetion, Lutedin, dissmetin - 7-O-β-D- Glucoside.
- Paederia foedita Linn. - Paederoside, Paederosidic acid, γ-Lactone, Rutin, 7-O-xylosil glucose, scandoside, Deacetyl asperuloside. ,,,
Guna - Guru; Rasa - Tikta; Vipaka - Katu; Virya - Ushna; Doshaghnata - Kapha-Vatashamaka 
Determination of Ash values
Ash values of both the plants was analysed for the present study at I.I.C.T., Hyderabad [Table 1].
The Colour Study was conducted for the present work at I.I.C.T., Hyderabad under ordinary light and U.V. light for different solvent extractions of both the plants [Table 2].
HPLC analysis of samples of both the plants was conducted for the present study at I.I.C.T., Hyderabad.
The samples of whole plant powders and Kwathas (decoction) of both the plants were taken for chromatographic analysis before the clinical study.
In this present study, a HPLC with a gradient system of pumps (LC-IOATvp of Shimadzu); a Photo-diode Array Detector PDA (SPD-M10 A vp Shimadzu) and a software based data processor (Class-LC 10 vp Shimadzu) has been used. The model has been included with built-in-validation features.
Instrumental parameters like pump flow and detector wavelength were calibrated. For injection a 7725i-rheodyne injector has been used with 20 micro liter loop.
The samples of whole plant powder of both plant were extracted into acqueous alcohol. Kwathas were prepared freshly from both the samples and were also taken for analysis.
In the present analysis, a gradient elusion with acetonitrile and water was done from 40 to 70% acetonitrile. A chromatogram was monitored at 225 nm. The contour chromatogram of PDA detector has helped to select a suitable wavelength for chromatography.
Observations and Results
The chromatograms were found to contain constituents having a general absorbance maxima at 225 nm. All the chromatograms of whole plant powders and Kwathas of both the samples were printed at same wavelengths. It was found that the whole plant powder sample of P. foetida has more number of constituents than that of M. tridentata. This indicates that P. foetida could have a better efficacy than M. tridentata.
The U.V. spectra of Kwatha of P. foetida sample showed a constituent at 4.1 minute which was not found in the M. tridentata Kwatha sample. It was observed that Kwatha of M. tridentata sample also contain a good number of constituents. Hence the efficacy could be almost equal to that of P. foetida. It was observed that there is a common constituent present in both the samples having same absorption spectra at 350 nm absorbance maxima indicating a common efficacy of both the plants.
| Discussion|| |
HPLC Studies: In the present study, even though a thorough chemical analysis was not conducted, the samples under study were analysed on HPLC which is a excellent method for analyzing the natural products.
The common constituents (having 350 mm absorbance maxima) indicates that both the plants M. tridentata and P. foetida work on a similar disease. Even though the constituents between 0 and 10 minutes are found to be similar, the presence of the constituents between 20 and 30 are more in the plant P. foetida than in M. tridentata and hence makes the difference. Chromatographical studies were helped to monitor the commonality of chemistry between both the plants.
In the M. tridentata sample, three molecules at 45 minutes were with λ - max at 280 and 310 nm which were found to be absent in the plant P. foetida. And even though both the plants contain common constituents absorbing at 225 nm, M. tridentata appears to have more number of constituents absorbing in the range of 250 to 350 nms. This indicates that the plant M. tridentata may show more Balya property than the plant P. foetida.
In the present chromatographic study, the analysis helped to know the difference of efficacy between the two samples due to the variation of chemical constituents. The present method may hence be useful for the quality control of the above two plant species.
By the results obtained, it is can be said that both the plants M. tridentata and P. foetida may be useful in treating the Vatarogas. It can be said that among the two, the plant P. foetida may be little more effective than the plant M. tridentata.
| Conclusion|| |
The plant Prasarani is in medicinal use since Samhita Period.
- HPLC studies helped to monitor the commonality of chemistry between both the plants
- It was observed that there is a common constituent (having 350 mm absorbance maxima) present in both the drug samples of M. tridentata and P. foetida indicates that both work on a similar disease.
- It was found that the whole plant powder sample of P. foetida has more number of constituents than that of M. tridentata. This indicates that P. foetida may have a better efficacy than M. tridentata.
| References|| |
|1.||Agnivesha, Charaka, Dridhabala, Charaka Samhita, Chikitsa Sthana, 9/47, 28/166; Vimana Sthana 8/135, edited by Yadavji Trikamji Achaya. Varanasi: Chaukhambha Surabharati Prakashan; 2000. |
|2.||Sushruta, Sushruta Samhita, Chikitsa Sthana 15/45-47 edited by Yadavji Trikamji Acharya. Varanasi: Chaukhambha Surabharati Prakashan; 2008. |
|3.||Vagbhata, Astanga Sangraha, Chikitsa Sthana 23/55, Uttarasthana 9/33, Pandit Rao DV, editor. Ayodhya Pandeya. New delhi: C.C.R.A.S. Publication. |
|4.||Bhavaprakasha, Bhavaprakasha Nighantu, editor - Chunekar KC. Hindi Commentary by Pandey GS. Chaukhambha Bharati Academy, Varanasi 2002; p. 424-5. |
|5.||Warrior PK. Indian Medicinal Plants - A Compendium of 500 species. Vol. 4, Chennai, Orient Longman; 2002. p. 22-6. |
|6.||Krishnappan AL, Seetharaman TR. Flavonoids of Merremia tridentate. Fitoterpia 1992;63:190. |
|7.||De S, Ravishankar B, Bhavasar GC. Preliminary phyto pharmacological studies on P.F.Linn. Indian J Pharm Sci 1990;52:63. |
|8.||Wong KC. Steam volatile constituents of the aerial parts of P.F. leaf. Medicinal & Aromatic Plant Abstract, 1994. |
|9.||Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol. 1-4, New Delhi: CDRI and Publication and Information Directorate, 1990. |
|10.||Sharma PV. Dravyaguna Vijnana, Part 2. Varanasi: Chaukhambha Bharati Academy; 2006. p. 62-4. |
[Figure 1], [Figure 2]
[Table 1], [Table 2]