Login   |  Users Online: 1605 Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
Search Article 
  
Advanced search 
   Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts


 
  Table of Contents  
PHARMACOLOGICAL RESEARCH
Year : 2011  |  Volume : 32  |  Issue : 3  |  Page : 388-394  

Antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits


1 Asst. Professor in Pharmaceutics, Department of Pharmaceutical Biotechnology, Mahatma Jyoti Rao Phoole College of Health Care and Allied Sciences, MJRP University, Jaipur, Rajasthan, India
2 Beharulal Mangilal College of Pharmaceutical Education and Research, Indore, Madhya Pradesh, India
3 Asst. Professor, Department of Pharmacognosy, Mahatma Jyoti Rao Phoole College of Health Care and Allied Sciences, MJRP University, Jaipur, Rajasthan, India
4 Medical Officer, Rani Dhullaiya Memorial Ayurvedic College and Hospital, Bhopal, Madhya Pradesh, India

Date of Web Publication17-Mar-2012

Correspondence Address:
Shaktikumar C Shivhare
Sadar Bazar, B/H Ram Mandir, Paratwada, Amaravati - 444 805, Maharashtra
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8520.93921

Rights and Permissions
   Abstract 

Several methods exist for the treatment of cancer in modern medicine. These include chemotherapy, radiotherapy, and surgery; most cancer chemotherapeutants severely affect the host normal cells. Hence the use of natural products now has been contemplated of exceptional value in the control of cancer. Plant-derived natural products such as flavonoids, terpenes, alkaloids, etc., have received considerable attention in recent years due to their diverse pharmacological properties including cytotoxic and cancer chemopreventive effects. Looking into this, the antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits has been attempted to investigate its antitumor activity. The collection and authentication of the plant material mainly fruits and their various extractions was done. Identification of plant's active constituents by preliminary phytochemical screening was carried out. An in-vitro cytotoxic assay using the brine shrimp lethality assay with brine shrimp eggs (Artemia salina) at a dose of 1-10 μg/ml with the fruit extract was performed by the method described by Mayer et al. Cell viability using the Trypan blue dye exclusion test at a dose of 20, 40, 80, 120, and 160 μg/ml dissolved in DMSO (final concentration 0.1%), and cytotoxicity using the MTT assay where viable cells convert MTT into a formazan salt were performed. All pharmacological screening for acute toxicity and anti tumour studies using EAC 1 x 10 6 cells/mouse treated Swiss albino mice at a dose of 100 and 200 mg/kg/day orally was carried out. Biochemical and antioxidants predictions from various parameters like hematological, RBC, WBC count, PVC, total protein, Tissue Lipid Peroxidation, SOD, CATALASE, GPx, GST levels and anti tumour activity of Scindapsus officinalis were observed. The data was statistically analyzed by one-way ANOVA followed by Dunnett's and Tukey's multiple comparison test. The antitumor effect of the extract is evident from the increase in mean survival time (MST) lifespan, reduction in the solid tumor volume, and also the reversal of altered hematological parameters almost equal to normal. The methanolic extract (100-200 mg/kg/day orally) was found to be cytotoxic on human cancer cell lines. In addition, the methanolic extract had an antioxidant effect as reflected by a decrease in LPO, GST, and GPx (oxidant enzymes), and an increase in SOD and catalase.

Keywords: Antioxidant, Ehrlich′s ascites carcinoma, hematological parameter, mean survival time, solid tumor volume, Scindapsus officinalis


How to cite this article:
Shivhare SC, Patidar AO, Malviya K G, Shivhare-Malviya K K. Antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits. AYU 2011;32:388-94

How to cite this URL:
Shivhare SC, Patidar AO, Malviya K G, Shivhare-Malviya K K. Antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits. AYU [serial online] 2011 [cited 2019 Nov 19];32:388-94. Available from: http://www.ayujournal.org/text.asp?2011/32/3/388/93921


   Introduction Top


Prevention is undeniably the sensible maneuver towards the ultimate goal of cancer control. Several methods exist for the treatment of cancer in modern medicine. These include chemotherapy, radiotherapy, and surgery. Chemotherapy is now considered as the most effective method of cancer treatment. Intervention with chemopreventive agents at the early stage in carcinogenesis is theoretically more rational than attempting to eradicate fully developed tumors with chemotherapeutic drugs. However, most cancer chemotherapeutants severely affect the host normal cells. Hence the use of natural products now has been contemplated as of exceptional value in the control of cancer and its eradication program. [1],[2] Plant-derived natural products such as flavonoids, terpenes, alkaloids, etc., have received considerable attention in recent years due to their diverse pharmacological properties including cytotoxic and cancer chemopreventive effects. Only few of them were scientifically explored. Scindapsus officinalis is a folklore medicinal plant used against diseases such as skin diseases and asthma; it causes flatulence, is good for curing ulcers, leprosy, nocturnal emissions, diabetes, and throat troubles, ophthalmia, tumors, and dysentery. It is alexetric, anthelmintic, and astringent. [3],[4],[5],[6],[7]

Hence, the antioxidant and anticancer evaluation of S. officinalis (Roxb.) Schott fruits is an attempt to investigate the antitumor activity against Ehrlich's ascites carcinoma in mice.

Scindapsus officinalis (Roxb.) Schott

Plant name
:[Figure 1]a Scindapsus officinalis, Class: Liliopsida, Subclass: Aridae Superorde: Aranae, Order: Alismatales, Family: Araceae
Figure 1: Scindapsus officinalis (a) plant and (b) fruits

Click here to view


Vernacular names: Gaj-pipali, Gajapipal, Atti-tippili, Enugutippali etc.

Synonyms : Monstera officinalis (Roxb.) Schott, Pothos officinalis Roxb., Scindapsus annamicus Gagnep. Latin: Pothos officinalis Roxb.

Botanical name : Scindapsus officinalis (Roxb.) Schott [4],[5],[6],[7],[8],[9]

Part useds: Fruit, Dried mature inflorescence, Shoots, Roots and Leaves.

Fruits: [Figure 1]b Fruits are the most important part of S. officinalis (Roxb.) Schott (Gaj-pipali) and accepted in both Unani and Ayurveda for its known properties. Fruits are pungent in taste, used to sharp hearing, regulating the bowels, appetizer, galactagogue, stimulant, carminative, diaphoretic, anthelmintic, antiprotozoal, antidiarrheal, intestinal colic in horse, tuberculosis, azoena, cancer, cholera, pneumonia, scabies, syphilis and bronchitis, hypoglycemic. Fruit decoction is used in asthma, bronchitis, diarrhea, and also as an expectorant. Fruit pulp is applied externally in rheumatism. The Ayurvedic Pharmacopoeia of India recommends dried pieces of mature female spadix in dyspnea. It is also considered as atonic and diuretic. The fruit of Gaj-pipali is an ingredient of preparation "Chandraprabha" tablets and "Ma'jun Jograj Gugul churna" which is used as a Nervine tonic and anticatarrhal. It is also useful in facial paralysis and general paralysis. The fruit of Gaj-pipali is an ingredient of the Ayurvedic preparation which is prescribed for Vatavikara, obesity (Medoroga and allied complaints), and obstinate urinary disorders including diabetes. It is useful in vitiated conditions of vata and kapha, diarrhea, cough, bronchitis, asthma, worm infestations, rheumatism, pharyngopathy, and helminthiasis. [4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15]


   Materials and Methods Top


Collection and authentication of the plant material

S. officinalis fruits were collected from Chennai, Tamil Nadu, India. They were identified and authenticated by a field botanist from Plant Anatomy Research Centre (PARC) (Tambaram, Chennai). The voucher specimen has been deposited at the herbarium unit of the Department of Pharmacognosy, Vel's College of Pharmacy, Pallavaram, Chennai.

Extraction of S. officinalis

The dried fruits were used for the preparation of the extract. The fruits were dried under shade and coarsely powdered and extracted with petroleum ether, chloroform, acetone, methanol, and water.

Identification of plant active constituents by preliminary phytochemical studies [Table 1]. All the extracts of dried fruits of S. officinalis (Roxb.) Schott were subjected for the identification of various active constituents, such as carbohydrates, glycosides. alkaloids, fixed oils and fats, proteins and free amino acids, saponins, phenolic compounds and tannins, gums and mucilages, flavonoids, and phytosterol.
Table 1: Preliminary phytochemical studies

Click here to view


In vitro cytotoxicity assay using brine shrimp and human cancer cell lines

Brine shrimp lethality and cytotoxicity assay


This assay uses brine shrimp, Artemia salina, which is used to determine the toxicity of the plant extract. Brine shrimp eggs of A. salina were hatched in artificial sea water (ASW; aqueous solution of NaCl, 3.8%w/v) and incubated at 25°C. The starting pH of the ASW was 8-8.5. After 48 h of hatching, the larvae (nauplii) were collected and used for brine shrimp lethality (BSL) bioassay. [16] The BSL assay of the successive leaf extract of plant materials was carried out by the method described by Mayer et al.[17],[18],[19],[20],[21] All the extracts and fractions were tested at concentration levels of 1-10 μg/ml. Each test was done in six replicates. A suspension of 10 nauplli (100 μl) was added into each well of a 24-well microplate and covered. The microplate was incubated for 24 h at room temperature. After this period, the number of dead nauplii in each well was counted using a binocular microscope. Potassium permanganate (100 μg/ml) was used as a standard (positive control) and a control reaction was carried out without the sample (negative control). The results were calculated statistically against their respective controls. The statistical method of probit analysis was used to calculate the concentration of the extract or fraction that would kill 50% of brine shrimps within the 24-h exposure, i.e., at the LC 50 value with the 95% confidence intervals. The extracts were considered bioactive when the LC 50 value was 1000 μg/ml or less [Table 2].
Table 2: Lethal dose (LC50) effect of different extracts of Scindapsus officinalis in the brine shrimp lethality bioassay

Click here to view


Cell cultures

Four human cancer cell lines were used for the present investigation. Acute myeloblastic leukemia (HL-60) and chronic myelogenic leukemia (K562) cells were maintained in RPMI1640 supplemented with the 15% heat inactivated fetal bovine serum and gentamycin (40 μg/ml), penicillin (100 units/ml), and streptomycin (10μg/ml). Breast adenocarcinoma (MCF7) and cervical epithelial carcinoma (HeLa) cells were maintained in MEM supplemented with similar concentrations of serum and antibiotics as stated above. Cells were grown at 37°C in a humidified atmosphere of 5% CO 2 /95% air.

Cell viability and cytotoxicity assay

Trypan blue dye exclusion


The viability of cells was determined by the trypan blue dye exclusion method and cytotoxicity was assessed by the MTT assay. [22],[23],[24] Exponentially growing cancer cell lines (1 × 10 4 ) were plated in 96-well plates and after 48 h of growth, the cells were treated with a series of concentrations of the various extracts of S. officinalis (20, 40, 80, 120, and 160 μg/ml dissolved in DMSO; final concentration 0.1%). Control cells were treated with DMSO alone and positive controls with various amounts of doxorubicin. Incubation was carried out at 37°C for 48 h. Cells were then exposed to 0.2% trypan blue and were counted in a hemocytometer [Figure 2] and [Figure 3]. The percentage of dead cells was calculated from which the IC 50 concentration was determined [Figure 2] and [Figure 3]:
Figure 2: Effect of MESO on cancer cell lines by the MTT assay

Click here to view
Figure 3: Effect of MESO on cancer cell lines by the cytotoxcity assay

Click here to view




MTT assay

MTT measures the metabolic activity of viable cells. The assay is nonradioactive which can be performed entirely in a microtiter plate (MTP). It is suitable for measuring cell proliferation, cell viability, and cytotoxicity. This method is based on the principle that viable cells convert MTT into a formazan salt, which is insoluble. It is solubilized and quantified. An increase in its concentration indicates an increased number of viable cells. The absorbance directly correlates with the cell number. This method is applicable to adherent cells cultured in MTP. [22],[23],[24] The MTT solution was added to each well (1.2 mg/ml) and incubation was done for 4 h. The reaction resulted in the reduction of MTT by mitochondrial dehydrogenases of viable cells to form a purple-colored formazan product. The formazan product was dissolved in DMSO and the amount was estimated by measuring absorbance at 570 nm in an ELISA plate reader. [25]

The percentage growth inhibition was calculated using the following formula:



Pharmacological screening

Acute toxicity study


In the acute toxicity study, the toxicity effect of the drug can be evaluated and the LD 50 and ED 50 values and the therapeutic index were determined for the drug under investigation.

Procedure

The up-and-down procedure was carried out as per the guidelines set by Organization for Economic Co-operation and Development (OECD). The chronic oral toxicity study was done according to OECD guideline 423. [26],[27] In this experiment, two groups of Wistar rats (n = 6) were used. Animals were kept on fast overnight with water ad libitum and food was withheld for 3-4 h after the oral administration of the extracts. One group of animals was treated with a starting dose of 1000 mg/kg b.wt. orally and the maximum dose of 2000 mg/kg b.wt. was administered to rats. Another group was treated with normal saline. The observation included mortality and clinical signs, which included changes in skin fur, eyes, and mucous membranes. The gross behaviors like body positions, locomotion, rearing, tremors, gait, passivity, grip strength, pain response, stereotypy, vocalization, righting reflex, body weight, and water intake were observed. From acute toxicity studies, it was observed that the administration of the methanol extract of S. officinalis to rats did not induce drug-related toxicity and mortality in the animals. The rats treated with the methanol extract of S. officinalis were well tolerated and exhibited normal behavior up to a dose of 2 g/kg orally. All animals were alert with normal grooming, touch response, and pain response and there was no sign of passivity, stereotypy, and vocalization. Their motor activity and secretary signs were also normal.

Anti-tumor activity of the S. officinalis methanolic extract

Animals used


Swiss albino mice (20-25 g) were used throughout the study after the clearance from the animal ethical committee (IX/290/CPCSEA/PHARMCOL/I-1.15/06). They were housed in standard microloan boxes and were given standard laboratory diet and water ad libitum.

Tumor cell lines

Ehrlich's ascites carcinoma (EAC) cells were obtained through the courtesy of Amala Cancer Research Centre (Thrissur, Kerala, India). EAC cells were maintained by weekly interaperitoneal (i.p.) inoculation of 1 × 10 6 cells/mouse.

Effect of the S. officinalis methanolic extract on survival time

Animals were inoculated with EAC 1 × 10 6 cells/mouse on day '0' and treatment with the S. officinalis methanolic extract (SME) started 24 h after administration, at the dose of 100 and 200 mg/kg/day orally. The control group was treated with the same volume of the 0.9% sodium chloride solution. All treatments were carried out for 9 days and observation was carried out for 45 days. The animals were subjected for the analysis of the median survival time (MST) of each group (n = 6) and changes in body weight. The antitumor efficacy of SME was compared with that of 5-fluorouracil (20 mg/kg/day i.p. for 9 days). MST was noted with reference to control [Table 3], [Figure 4] and [Figure 5]. Survival times of the treated group (T) were compared with those of the control groups (C) using the following calculation:



where T is the number of days treated animals survived and C is the number of days control animal survived. [28],[29],[30],[31],[32]
Figure 4: Effect of MESO treatment on the survival of tumor-bearing mice

Click here to view
Figure 5: Effect of MESO treatment on the percentage of lifespan of tumor-bearing mice

Click here to view
Table 3: Effect of MESO treatment on the survival of tumor-bearing mice

Click here to view


Effect of SME on hematological parameters

In order to detect the influence of SME on the hematological status of EAC-bearing mice, a comparison was made among four groups (n = 6) of mice on the 14th day after administration. The four groups comprised (1) control mice, (2) control + tumor bearing mice, (3) tumor-bearing mice treated with SME 100 mg/1 kg, and (4) tumor-bearing mice treated with SME 200 mg/1 kg. Blood was drawn from each mouse by the saphenous vein puncture method and the white blood cell count (WBC), red blood cell count (RBC), hemoglobin (Hb), protein, and packed cell volume (PCV) were determined [Table 4], [Table 5] and [Figure 6]. The ascetic fluids were collected on the 14th day and smeared. The smear was stained with the Giemsa stain for cytological studies. The liver was removed and preserved in the Tris-HCI buffer (pH 7.4). A 10% liver homogenate was used for the measurement of oxidative stress markers and antioxidants such as tissue lipid peroxidation (LPO), superoxide dismutase (SOD) catalase, glutathione peroxidase (GPX), and glutathione S-transferase (GST). [16],[21],[25],[33],[34],[35]
Figure 6: Effect of MESO on the antioxidant level in EAC-induced tumor-bearing mice

Click here to view
Table 4: Effect of MESO on the antioxidant level in EAC - induced tumor - bearing mice

Click here to view
Table 5: Effect of MESO on hematological parameters in tumor - bearing mice

Click here to view



   Results and Discussion Top


The present investigation was aimed to carry out the possible in vivo BSL bioassay of various successive fruit extracts of S. officinalis as a preliminary bioactive index; furthermore, the successive leaf extracts were investigated for in vitro cytotoxicity in various human cancer cell lines because scientific scrutiny of therapeutic potential, biological properties, and safety profile of S. officinalis will be useful in resuming further experiments. In the present study, the methanol extract was active in the BSL assay at the lethal dose (LC 50 ) concentration of 10-100 μg/ml. Brine shrimps have been used for the screening of pesticides, industrial toxins, opioids, antitumor agents, and antibiotics. The BSL assay is an index of a bidirectional biological activity, which suggests the bioactivity of the plant extract as well as the cytotoxic activity of the plant material tested. Furthermore, our work has been directed toward screening the cytotoxicity of the successive fruit extracts of S. officinalis in four different carcinoma cell lines. Among all the extracts, the ethyl acetate and methanol extracts of the plant possess selective cytotoxicity, which were found to be more effective in leukemic cell lines and were less effective in MCF-7 and HeLa. The IC 50 value of the methanol extract was found to be 33.11 and 39.81 μg/ml. However, the extract did not show any cytotoxicity in normal cell lines (data not shown). The above findings revealed that the extract of methanol possesses significant inhibition against the BSL bioassay and cytotoxic effect in acute myeloblastic leukemia (HL-60) and chronic myelogenic leukemia (K-562) cell lines. This activity is due to the complex interplay of the varied phytoconstituents present in the methanol extract. Further preliminary phytochemical investigation of the methanol extract suggests the presence of phytoconstituents like flavonoids, tannins, and glycosides which prompted us to screen the anticancer potential of the methanol extract in EAC-bearing mice. The antitumor effect of the MESO extract is evident from the increase in the mean survival time and percentage of increase in lifespan, and also the reversal of the altered hematological parameter almost equal to normal. The reliable criteria for evaluating an anticancer drug are the prolongation of lifespan of the animal and a decrease in the WBC count of blood. Our results show an increase in lifespan accompanied by a reduction in the WBC count in MESO-treated mice. These results clearly demonstrate the antitumor effect of MESO against EAC. The common problems encountered in cancer chemotherapy are myelosuppression and anemia. Anemia occurring in tumor-bearing mice is mainly due to the reduction in RBC or hemoglobin production, and this may occur either due to iron deficiency or due to hemolytic or other myelopathic conditions. Treatment with APHE brought back the hemoglobin content, and RBC and WBC counts to near normal. This indicates that MESO has a protective effect on the hemopoietic system. The free radical scavenging system, SOD, and catalase are present in all oxygen-metabolizing cells and their function is to provide a defense against the potentially damaging reactivates of superoxide and hydrogen peroxide. The observed decrease in the SOD activity in EAC-bearing mice might be due to the loss of the Mn SOD activity in EAC cells and loss of mitochondria, leading to a decrease in the total SOD activity in the liver. The inhibition of SOD and CAT activities as a result of tumor growth was also reported. Similar findings were observed in the present investigation with EAC-bearing mice.

An excessive production of free radicals results in oxidative stress, which leads to the damage of macromolecules such as lipids and can induce lipid peroxidation in vivo. Increased lipid peroxidation would cause the degeneration of tissues. Lipid peroxide formed in the primary site would be transferred through the circulation and provoke damage by propagating the process of lipid peroxodation. MDA, the end-product of lipid peroxidation, was reported to be higher in carcinomatous tissues than in nondiseased organs. Glutathione, a potent inhibitor of the neoplastic process, plays an important role as an endogenous antioxidant system that is found particularly in high concentrations in the liver and is known to have key function in the protective process. Plant-derived extracts containing antioxidant principles showed cytotoxicity toward tumor cells and an antitumor activity in experimental animals. The lowering of lipid peroxidation, GST, and GPX, and an increase in levels of SOD and catalase in APHE-treated groups indicate its potential as an inhibitor of EAC-induced intracellular oxidative stress.


   Conclusion Top


It can be concluded that the ethyl acetate and methanol extract from the fruits of S. officinalis possesses an antitumor activity, which is confirmed by the brine shrimp lethality bioassay, various human carcinoma cell lines, and the rodent model of cancer. The biochemical investigational report of lipid peroxidation, GST, GPX, SOD, and catalase levels also shows that the plant possesses an antioxidant activity. Further investigations have to be carried out in isolation and the characterization of active constituents and the mechanism involved in the antitumor and cytotoxic effect is needed.

 
   References Top

1.Itharat A, Peter J. Raman A. In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer. J Ethnopharmacol 2004;90:33-8.  Back to cited text no. 1
    
2.Wilson KA. The genera of the Arales in the southeastern United States. J Arnold Arbor 1960;41:47-72.  Back to cited text no. 2
    
3.Babu TD, Kuttan G, Padikkala J. Cytotoxic and anti-tumor properties of certain taxa of Umbelliferae with special reference to Centella asiatica (L.) Urban. J Ethnopharmacol 1995;48:53-7.  Back to cited text no. 3
    
4.Shah CS, Quadry JS. A text of Pharmacognosy. Pune: Nirali Prakashan; 2005. p. 136-45.  Back to cited text no. 4
    
5.Charlson AJ. Antineoplastic constituents of some southern African plants. J Ethnopharmacol 1980;2:323-35.  Back to cited text no. 5
    
6.Velraj M, Singh M, Ravichandiran V, Jayakumari S, Ragela S. Free radical scavenging activity of Scindapsus officinalis Fruits. Res J Pharmacogn Phytochem 2010;2:280-3.  Back to cited text no. 6
    
7.Dr. Rangari VD. "Pharmacognosy and Phytochemistry" Part 1, 1st ed. Pune: Career Publication; 2003.  Back to cited text no. 7
    
8.Shivhare Y, Singh P, Gadekar R, Soni P. Botanicals as antioxidants: A renovate review. Res J Pharmacogn Phytochem 2010;2:255-9.  Back to cited text no. 8
    
9.Elangovan V, Ramamoorthy N, Balasubramanian S. Studies on the antiproliferative effect of some naturally occurring bioflavonoidal compounds against human carcinoma of larynx and sarcoma-180 cell lines. Indian J Pharmacol 1994;26:266-9.   Back to cited text no. 9
  Medknow Journal  
10.Kokate CK, Purohit AP, Gokhale SB. " Pharmacognosy" 1st ed. Pune: Nirali Prakashan; 1990. p. 23  Back to cited text no. 10
    
11.Rao KN, Bhattacharya RK. Inhibition of thymidylate synthase and cell growth by the phenanthroindolizidine alkaloids pergularinine and tylophorinidine. Chem Biol Interact 1997;106:201-12.  Back to cited text no. 11
    
12.Sharma PK, Vijayan P, Dhanaraj SA, Suresh B. Cytotoxity studies on some spesies of Hypericum. Indian Drugs 2006;43:307-14.  Back to cited text no. 12
    
13.Singhal GD, Patterson TJ. Synopsis of ayurveda. 1st ed. Integrative Med 2002;1:10-22.  Back to cited text no. 13
    
14.Zhou BN. Some progress on the chemistry of natural bioactive terpenoids from Chinese medicinal plants. Mem Inst Oswaldo Cruz 1991;86:219-26.  Back to cited text no. 14
    
15.Brown JP. A rewiew of genetic effects of occurring Flavonoids, anthraquinones and related compounds. Mutat Res 1980;75:243-77.  Back to cited text no. 15
    
16.Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. A convenient general bioassay for active plant constituents. Planta Medica 1982;45:31-4.  Back to cited text no. 16
    
17.Carballo JL, Hernández ZL, Pérez P, García-Grávalos MD. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnol 2002;2:17.  Back to cited text no. 17
    
18.Pelka M, Danzl C, Distler W, Petschelt A. A new screening test toxicity testing of dental materials. J Dent 2000;28:341-5.  Back to cited text no. 18
    
19.Lewis GE. Testing the toxicity of extracts of Southern African plants using brine shrimp (Artemia salina). S Afr J Sci 1995;91:382-4.  Back to cited text no. 19
    
20.Annie S, Rajagopal PL, Malini A. Effect of Cassia auriculata Linn. root extract on cisplatin and gentamicin-induced renal injury. Phytomedicine 2005;12:555-60.  Back to cited text no. 20
    
21.Christina AJ, Joseph DG, Packialakshmi M, Kothai R, Robert SJ, Chidambaranathan N, et al. Anticarcinogenic activity of Withanai somnifera dunal against Dalton's Ascitic Lymphoma. J Ethnopharmacol 2004;93:359-61.  Back to cited text no. 21
    
22.Dekordi AJ, Emami SA, Saeidi M, Sadeghi H. Cytotoxicologic studies of the extracts of Iranian Juniperus Sabina and Platycladus orentalis on cancer cells. J Res Med Sci 2004;5:7-11.  Back to cited text no. 22
    
23.Patel S, Gheewala N, Suthar R, Shah A. In-Vitro cytotoxicity activity of Solanum Nigrum extract against Hela cell line and Vero cell line. Int J Pharm Pharm Sci 2009;1:38-46.  Back to cited text no. 23
    
24.Gupta M, Gupta AK. In vitro cytotoxicity studies of hydrogel pullulan nanoparticles preparedbyaot/n-hexan emicellar system. J Pharm Pharm Sci 2004;7:38-46.  Back to cited text no. 24
    
25.McLaughlin JL. Methods in Plant Biochemistry. In: Hostettmann K, editor. Assays for Bioactivity. Vol. 6. London: Academic Press; 1991. p. 1-33.  Back to cited text no. 25
    
26.Lindholm P, Gullobo J, Claeson P, Goransson. U, Johansson S, Backlund A, et al. Selective cytotoxic evaluation in anticancer drugs screening of fractionated plant extracts J Biomol Screen 2002;7:333-40.  Back to cited text no. 26
    
27.Croce CM. "Oncogenes and cancer". N Engl J Med 2008;358:502-11.  Back to cited text no. 27
    
28.Banerjee S, Prashar R, Kumar A, Rao AR. Modulatory influence of alcoholic extract of Ocimum leaves on Carcinogen- metabolizing enzyme activities and reduced glutathione levels in mouse. Nutr Cancer 1996;25:205-17.  Back to cited text no. 28
    
29.Jageti GC, Rao SK. Evaluation of the antineoplastic activity of Guduchi (Tinospora cordifolia) in Ehrlich ascites carcinoma in bearing mice. J Biol Pharm Bull 2006;29:460-6.  Back to cited text no. 29
    
30.Knanam JA, Bag SP. Antineoplastic activity of copper benzohydroxamic acid complex against Erhlich Ascitic carcinoma in mice. Indian J Pharmacol 1997;29:157-61.  Back to cited text no. 30
    
31.Mazumdar UK, Gupta M, Maiti S, Mukherjee D. Antitumor activity of Hygrophila spinosa on Ehrlich ascites carcinoma and sarcoma-180 induced mice. Indian J Exp Biol 1997;35:473-7.  Back to cited text no. 31
    
32.Rosangki G, Prasad SB. Antitumour activity of some plants from Meghalaya against murine Ehrlich Ascites Carcinoma. Indian J Exp Biol 2004;42:981-8.  Back to cited text no. 32
    
33.Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival: Modifi cations to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 1986;89:271-7.  Back to cited text no. 33
    
34.Miyake Y, Yamamoto K, Tsujihara N, Osawa T. Protective effects of lemon flavonoids on oxidative stress in diabetic rats Lipids 1998;33:689-95.  Back to cited text no. 34
    
35.Leeuwenburgh C, Ji LL. Glutathione depletion in rested and exercised mice: Biochemical consequence and adaptation. Arch Biochem Biophys 1995;316:941-9.  Back to cited text no. 35
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 6]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Figure 5]


This article has been cited by
1 Phenolic cyclobutantetraol esters from Scindapsus officinalis (Roxb.) Schott
Jinqian Yu,Xiaowei Sun,Yan Mu,Xingang Shi,Xueyong Wang,Xiao Wang
Fitoterapia. 2019; 137: 104244
[Pubmed] | [DOI]
2 Total Phenolic Content and DPPH Free Radical Scavenging Activity of Young Turmeric Grown in Southern Thailand
Suriyan Sukati,Warachate Khobjai
Applied Mechanics and Materials. 2019; 886: 61
[Pubmed] | [DOI]
3 Ethyl Acetate Extract of Scindapsus cf. hederaceus Exerts the Inhibitory Bioactivity on Human Non-Small Cell Lung Cancer Cells through Modulating ER Stress
Chon-Kit Chou,Wangta Liu,Yu-Jie Hong,Hans-Uwe Dahms,Chen-Hao Chiu,Wen-Tsan Chang,Ching-Ming Chien,Chia-Hung Yen,Yuan-Bin Cheng,Chien-Chih Chiu
International Journal of Molecular Sciences. 2018; 19(7): 1832
[Pubmed] | [DOI]
4 Ethnobotanical and phytopharmacological review of Scindapsus officinalis (“Gajapippali”)
Kuljeet Kaur,Rajiv Gupta
Asian Pacific Journal of Tropical Biomedicine. 2017; 7(1): 78
[Pubmed] | [DOI]
5 Thione-Based Nickel(II) Complexes as Functional Antioxidant Mimics: Scavenging Activity of Reactive Oxygen Species O2 -· and X-Ray Crystal Structure of [Ni(Ttxyly)2]2 {Ttxylyl?=?Hydrotris(2-mercapto-1-xylyl-imidazolyl)borate}
Mohamed M. Ibrahim,Abd El-Motaleb M. Ramadan,Shaban Y. Shaban,Gaber A. M. Mersal,Mohamed M. Soliman,Salih Al-Juaid
Journal of Inorganic and Organometallic Polymers and Materials. 2017;
[Pubmed] | [DOI]
6 Antitumor Potential of Ethyl Acetate Extract of Kirganelia reticulata Against Ehrlich Ascites Carcinoma Tumor
A.R. Kharat,Yogita S. Tarkasband,Kiran Kharat
Journal of Biologically Active Products from Nature. 2016; 6(4): 337
[Pubmed] | [DOI]
7 In-vitro Anthelmintic Efficacy of Aqueous Extract of Scindapsus officinalis (Roxb.) Schott Fruits Against Haemonchus contortus of Small Ruminants
Gagandeep Singh,Rajeev Singh,Pawan Kumar Verma,Rajiv Singh,Atul Anand
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2014;
[Pubmed] | [DOI]



 

Top
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
   Introduction
    Materials and Me...
    Results and Disc...
   Conclusion
    References
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed2967    
    Printed108    
    Emailed0    
    PDF Downloaded517    
    Comments [Add]    
    Cited by others 7    

Recommend this journal